Biopterin status in dogs with myxomatous mitral valve disease is associated with disease severity and cardiovascular risk factors
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Biopterin status in dogs with myxomatous mitral valve disease is associated with disease severity and cardiovascular risk factors. / Reimann, Maria Josefine; Häggström, J.; Mortensen, Alan; Lykkesfeldt, Jens; Møller, Jacob Eifer; Falk, Bo Torkel; Olsen, Lisbeth Høier.
I: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, Bind 28, Nr. 5, 2014, s. 1520-1526.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Biopterin status in dogs with myxomatous mitral valve disease is associated with disease severity and cardiovascular risk factors
AU - Reimann, Maria Josefine
AU - Häggström, J.
AU - Mortensen, Alan
AU - Lykkesfeldt, Jens
AU - Møller, Jacob Eifer
AU - Falk, Bo Torkel
AU - Olsen, Lisbeth Høier
N1 - Copyright © 2014 by the American College of Veterinary Internal Medicine.
PY - 2014
Y1 - 2014
N2 - BACKGROUND: Endothelial dysfunction (ED) has been suggested to be associated with myxomatous mitral valve disease (MMVD) in dogs. Tetrahydrobiopterin (BH4) is an important cofactor for production of the endothelium-derived vasodilator nitric oxide (NO). Under conditions of oxidative stress, BH4 is oxidized to the biologically inactive form dihydrobiopterin (BH2). Thus, plasma concentrations of BH2 and BH4 may reflect ED and oxidative stress.OBJECTIVE: To determine plasma concentrations of BH2 and BH4 in dogs with different degrees of MMVD.ANIMALS: Eighty-four privately owned dogs grouped according to ACVIM guidelines (37 healthy control dogs including 13 Beagles and 24 Cavalier King Charles Spaniels [CKCSs], 33 CKCSs with MMVD of differing severity including 18 CKCSs [group B1] and 15 CKCSs [group B2], and 14 dogs of different breeds with clinical signs of congestive heart failure [CHF] because of MMVD [group C]).METHODS: Dogs underwent clinical examination including echocardiography. Plasma concentrations of BH2 and BH4 were measured using high-performance liquid chromatography with fluorescence detection.RESULTS: Higher plasma BH4 and BH2 concentrations were found with dogs in CHF compared with all other groups (control, B1 and B2; P ≤ .001). Females had higher concentrations of BH4 and BH4/BH2 (P ≤ .0003). BH4/BH2 was found to decrease with age (P < .0001). Cardiovascular risk factors in humans such as passive smoking (P ≤ .01) and increased body weight (P ≤ .009) were associated with lower BH4 concentrations.CONCLUSIONS AND CLINICAL IMPORTANCE: Age, sex, body weight, passive smoking, and cardiac status are associated with plasma biopterin concentration in dogs. Additional studies should clarify the clinical implications of the findings.
AB - BACKGROUND: Endothelial dysfunction (ED) has been suggested to be associated with myxomatous mitral valve disease (MMVD) in dogs. Tetrahydrobiopterin (BH4) is an important cofactor for production of the endothelium-derived vasodilator nitric oxide (NO). Under conditions of oxidative stress, BH4 is oxidized to the biologically inactive form dihydrobiopterin (BH2). Thus, plasma concentrations of BH2 and BH4 may reflect ED and oxidative stress.OBJECTIVE: To determine plasma concentrations of BH2 and BH4 in dogs with different degrees of MMVD.ANIMALS: Eighty-four privately owned dogs grouped according to ACVIM guidelines (37 healthy control dogs including 13 Beagles and 24 Cavalier King Charles Spaniels [CKCSs], 33 CKCSs with MMVD of differing severity including 18 CKCSs [group B1] and 15 CKCSs [group B2], and 14 dogs of different breeds with clinical signs of congestive heart failure [CHF] because of MMVD [group C]).METHODS: Dogs underwent clinical examination including echocardiography. Plasma concentrations of BH2 and BH4 were measured using high-performance liquid chromatography with fluorescence detection.RESULTS: Higher plasma BH4 and BH2 concentrations were found with dogs in CHF compared with all other groups (control, B1 and B2; P ≤ .001). Females had higher concentrations of BH4 and BH4/BH2 (P ≤ .0003). BH4/BH2 was found to decrease with age (P < .0001). Cardiovascular risk factors in humans such as passive smoking (P ≤ .01) and increased body weight (P ≤ .009) were associated with lower BH4 concentrations.CONCLUSIONS AND CLINICAL IMPORTANCE: Age, sex, body weight, passive smoking, and cardiac status are associated with plasma biopterin concentration in dogs. Additional studies should clarify the clinical implications of the findings.
KW - Faculty of Health and Medical Sciences
KW - Endothelial dysfunction
KW - Mitral regurgitation
KW - Oxidative stress
KW - Tetrahydrobiopterin
U2 - 10.1111/jvim.12425
DO - 10.1111/jvim.12425
M3 - Journal article
C2 - 25274442
VL - 28
SP - 1520
EP - 1526
JO - Journal of Veterinary Internal Medicine
JF - Journal of Veterinary Internal Medicine
SN - 0891-6640
IS - 5
ER -
ID: 124618815