Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats

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Standard

Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats. / Christensen, Sarah Louise T; Petersen, Steffen; Sørensen, Dorte Bratbo; Jansen-Olesen, Inger.

I: Journal of Pharmacological and Toxicological Methods, Bind 80, 2016, s. 43-50.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Christensen, SLT, Petersen, S, Sørensen, DB & Jansen-Olesen, I 2016, 'Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats', Journal of Pharmacological and Toxicological Methods, bind 80, s. 43-50. https://doi.org/10.1016/j.vascn.2016.04.004

APA

Christensen, S. L. T., Petersen, S., Sørensen, D. B., & Jansen-Olesen, I. (2016). Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats. Journal of Pharmacological and Toxicological Methods, 80, 43-50. https://doi.org/10.1016/j.vascn.2016.04.004

Vancouver

Christensen SLT, Petersen S, Sørensen DB, Jansen-Olesen I. Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats. Journal of Pharmacological and Toxicological Methods. 2016;80:43-50. https://doi.org/10.1016/j.vascn.2016.04.004

Author

Christensen, Sarah Louise T ; Petersen, Steffen ; Sørensen, Dorte Bratbo ; Jansen-Olesen, Inger. / Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats. I: Journal of Pharmacological and Toxicological Methods. 2016 ; Bind 80. s. 43-50.

Bibtex

@article{d900489606b644adadee0c1af516c4d6,
title = "Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats",
abstract = "Introduction: Glyceryl trinitrate induces headache during infusion to man and migraine patients develop an additional migraine attack a few hours after the infusion. Recently, we have moved this model into rat with the intention of developing an animal model predictive of migraine therapy. In the current paper we have studied the effect of glyceryl trinitrate infusion on three different rat behaviors. Methods: The stability of burrowing behavior, running wheel activity and light sensitivity towards repeated testing was evaluated also with respect to estrous cycle. Finally, the effect of glyceryl trinitrate on these behaviors in female rats was observed. Results: Burrowing behavior and running wheel activity were stable in the individual rat between experiments. The burrowing behavior was significantly affected by the stage of estrous cycle. The other assays were stable throughout the cycle. None of the three behavioral tests were altered by glyceryl trinitrate infusion. In the light-dark box, some batches of rats showed light sensitivity after treatment with glyceryl trinitrate but it could not be repeated in other batches of rats. Discussion: We have investigated the stability towards repeated testing and the effect of i.v. glyceryl trinitrate infusion to awake rats in three behavioral assays. Of the assays evaluated, only light sensitivity was capable of detecting changes after glyceryl trinitrate infusion but, this was not repeatable. Thus, the infusion of a low dose glyceryl trinitrate to concious rats together with the chosen behavioral tests is not a robust setup for studying immediate GTN induced headache behavior in rats",
keywords = "Faculty of Health and Medical Sciences, Animal model, Burrowing, Glyceryl trinitrate, Light sensitivity, Methods, Rat, Running wheel, Oestrous cycle, Migraine",
author = "Christensen, {Sarah Louise T} and Steffen Petersen and S{\o}rensen, {Dorte Bratbo} and Inger Jansen-Olesen",
year = "2016",
doi = "10.1016/j.vascn.2016.04.004",
language = "English",
volume = "80",
pages = "43--50",
journal = "Journal of Pharmacological and Toxicological Methods",
issn = "1056-8719",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Infusion of low dose glyceryl trinitrate has no consistent effect on burrowing behavior, running wheel activity and light sensitivity in female rats

AU - Christensen, Sarah Louise T

AU - Petersen, Steffen

AU - Sørensen, Dorte Bratbo

AU - Jansen-Olesen, Inger

PY - 2016

Y1 - 2016

N2 - Introduction: Glyceryl trinitrate induces headache during infusion to man and migraine patients develop an additional migraine attack a few hours after the infusion. Recently, we have moved this model into rat with the intention of developing an animal model predictive of migraine therapy. In the current paper we have studied the effect of glyceryl trinitrate infusion on three different rat behaviors. Methods: The stability of burrowing behavior, running wheel activity and light sensitivity towards repeated testing was evaluated also with respect to estrous cycle. Finally, the effect of glyceryl trinitrate on these behaviors in female rats was observed. Results: Burrowing behavior and running wheel activity were stable in the individual rat between experiments. The burrowing behavior was significantly affected by the stage of estrous cycle. The other assays were stable throughout the cycle. None of the three behavioral tests were altered by glyceryl trinitrate infusion. In the light-dark box, some batches of rats showed light sensitivity after treatment with glyceryl trinitrate but it could not be repeated in other batches of rats. Discussion: We have investigated the stability towards repeated testing and the effect of i.v. glyceryl trinitrate infusion to awake rats in three behavioral assays. Of the assays evaluated, only light sensitivity was capable of detecting changes after glyceryl trinitrate infusion but, this was not repeatable. Thus, the infusion of a low dose glyceryl trinitrate to concious rats together with the chosen behavioral tests is not a robust setup for studying immediate GTN induced headache behavior in rats

AB - Introduction: Glyceryl trinitrate induces headache during infusion to man and migraine patients develop an additional migraine attack a few hours after the infusion. Recently, we have moved this model into rat with the intention of developing an animal model predictive of migraine therapy. In the current paper we have studied the effect of glyceryl trinitrate infusion on three different rat behaviors. Methods: The stability of burrowing behavior, running wheel activity and light sensitivity towards repeated testing was evaluated also with respect to estrous cycle. Finally, the effect of glyceryl trinitrate on these behaviors in female rats was observed. Results: Burrowing behavior and running wheel activity were stable in the individual rat between experiments. The burrowing behavior was significantly affected by the stage of estrous cycle. The other assays were stable throughout the cycle. None of the three behavioral tests were altered by glyceryl trinitrate infusion. In the light-dark box, some batches of rats showed light sensitivity after treatment with glyceryl trinitrate but it could not be repeated in other batches of rats. Discussion: We have investigated the stability towards repeated testing and the effect of i.v. glyceryl trinitrate infusion to awake rats in three behavioral assays. Of the assays evaluated, only light sensitivity was capable of detecting changes after glyceryl trinitrate infusion but, this was not repeatable. Thus, the infusion of a low dose glyceryl trinitrate to concious rats together with the chosen behavioral tests is not a robust setup for studying immediate GTN induced headache behavior in rats

KW - Faculty of Health and Medical Sciences

KW - Animal model

KW - Burrowing

KW - Glyceryl trinitrate

KW - Light sensitivity

KW - Methods

KW - Rat

KW - Running wheel

KW - Oestrous cycle

KW - Migraine

U2 - 10.1016/j.vascn.2016.04.004

DO - 10.1016/j.vascn.2016.04.004

M3 - Journal article

C2 - 27066766

VL - 80

SP - 43

EP - 50

JO - Journal of Pharmacological and Toxicological Methods

JF - Journal of Pharmacological and Toxicological Methods

SN - 1056-8719

ER -

ID: 165395811