Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins

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Andreas Hougaard Laustsen, Brian Lohse, Arne Redsted Rasmussen

The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP- HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identi- fied or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2 (13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/ vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, L-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a ‘toxicity score’, were a-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical a-neurotoxins were obtained. As expected, no neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide pros- pects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening.
OriginalsprogEngelsk
TidsskriftToxicon
Vol/bind99
Sider (fra-til)23-35
Antal sider13
ISSN0041-0101
DOI
StatusUdgivet - 2015

ID: 134705042