Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata

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Standard

Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata. / Barslund, Anne Fuglsang; Poulsen, Mette Homann; Bach, Tinna Brøbech; Lucas, Simon; Kristensen, Anders Skov; Strømgaard, Kristian.

I: Journal of Natural Products, Bind 74, Nr. 3, 2011, s. 483-486.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Barslund, AF, Poulsen, MH, Bach, TB, Lucas, S, Kristensen, AS & Strømgaard, K 2011, 'Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata', Journal of Natural Products, bind 74, nr. 3, s. 483-486. https://doi.org/10.1021/np100746w

APA

Barslund, A. F., Poulsen, M. H., Bach, T. B., Lucas, S., Kristensen, A. S., & Strømgaard, K. (2011). Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata. Journal of Natural Products, 74(3), 483-486. https://doi.org/10.1021/np100746w

Vancouver

Barslund AF, Poulsen MH, Bach TB, Lucas S, Kristensen AS, Strømgaard K. Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata. Journal of Natural Products. 2011;74(3):483-486. https://doi.org/10.1021/np100746w

Author

Barslund, Anne Fuglsang ; Poulsen, Mette Homann ; Bach, Tinna Brøbech ; Lucas, Simon ; Kristensen, Anders Skov ; Strømgaard, Kristian. / Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata. I: Journal of Natural Products. 2011 ; Bind 74, Nr. 3. s. 483-486.

Bibtex

@article{8ec69fd5b0a0443ca674b5d55ee0a2c9,
title = "Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata",
abstract = "Polyamine toxins from orb weaver spiders are attractive pharmacological tools particularly for studies of ionotropic glutamate (iGlu) receptors in the brain. These polyamine toxins are biosynthesized in a combinatorial manner, providing a plethora of related, but structurally complex toxins to be exploited in biological studies. Here, we have used solid-phase synthetic methodology for the efficient synthesis of Joro spider toxin-4 (JSTX-4) (1) from Nephila clavata, providing sufficient amounts of the toxin for biological evaluation at iGlu receptor subtypes using electrophysiology. Biological evaluation revealed that JSTX-4 inhibits iGlu receptors only in high µM concentrations, thereby being substantially less potent than structurally related polyamine toxins.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Barslund, {Anne Fuglsang} and Poulsen, {Mette Homann} and Bach, {Tinna Br{\o}bech} and Simon Lucas and Kristensen, {Anders Skov} and Kristian Str{\o}mgaard",
year = "2011",
doi = "10.1021/np100746w",
language = "English",
volume = "74",
pages = "483--486",
journal = "Journal of Natural Products",
issn = "0163-3864",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Solid-phase synthesis and biological evaluation of Joro spider toxin-4 from Nephila clavata

AU - Barslund, Anne Fuglsang

AU - Poulsen, Mette Homann

AU - Bach, Tinna Brøbech

AU - Lucas, Simon

AU - Kristensen, Anders Skov

AU - Strømgaard, Kristian

PY - 2011

Y1 - 2011

N2 - Polyamine toxins from orb weaver spiders are attractive pharmacological tools particularly for studies of ionotropic glutamate (iGlu) receptors in the brain. These polyamine toxins are biosynthesized in a combinatorial manner, providing a plethora of related, but structurally complex toxins to be exploited in biological studies. Here, we have used solid-phase synthetic methodology for the efficient synthesis of Joro spider toxin-4 (JSTX-4) (1) from Nephila clavata, providing sufficient amounts of the toxin for biological evaluation at iGlu receptor subtypes using electrophysiology. Biological evaluation revealed that JSTX-4 inhibits iGlu receptors only in high µM concentrations, thereby being substantially less potent than structurally related polyamine toxins.

AB - Polyamine toxins from orb weaver spiders are attractive pharmacological tools particularly for studies of ionotropic glutamate (iGlu) receptors in the brain. These polyamine toxins are biosynthesized in a combinatorial manner, providing a plethora of related, but structurally complex toxins to be exploited in biological studies. Here, we have used solid-phase synthetic methodology for the efficient synthesis of Joro spider toxin-4 (JSTX-4) (1) from Nephila clavata, providing sufficient amounts of the toxin for biological evaluation at iGlu receptor subtypes using electrophysiology. Biological evaluation revealed that JSTX-4 inhibits iGlu receptors only in high µM concentrations, thereby being substantially less potent than structurally related polyamine toxins.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1021/np100746w

DO - 10.1021/np100746w

M3 - Journal article

C2 - 21188966

VL - 74

SP - 483

EP - 486

JO - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

IS - 3

ER -

ID: 32318999