Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray. / Skottrup, Peter Durand; Hansen, Mikkel Fougt; Moresco, Jacob Lange; Deryabina, Maria; Svendsen, Winnie Edith; Jakobsen, Mogens Havsteen; Dufva, Martin.

I: Acta Biomaterialia, Bind 6, Nr. 10, 2010, s. 3936-3946.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Skottrup, PD, Hansen, MF, Moresco, JL, Deryabina, M, Svendsen, WE, Jakobsen, MH & Dufva, M 2010, 'Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray', Acta Biomaterialia, bind 6, nr. 10, s. 3936-3946.

APA

Skottrup, P. D., Hansen, M. F., Moresco, J. L., Deryabina, M., Svendsen, W. E., Jakobsen, M. H., & Dufva, M. (2010). Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray. Acta Biomaterialia, 6(10), 3936-3946.

Vancouver

Skottrup PD, Hansen MF, Moresco JL, Deryabina M, Svendsen WE, Jakobsen MH o.a. Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray. Acta Biomaterialia. 2010;6(10):3936-3946.

Author

Skottrup, Peter Durand ; Hansen, Mikkel Fougt ; Moresco, Jacob Lange ; Deryabina, Maria ; Svendsen, Winnie Edith ; Jakobsen, Mogens Havsteen ; Dufva, Martin. / Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray. I: Acta Biomaterialia. 2010 ; Bind 6, Nr. 10. s. 3936-3946.

Bibtex

@article{85bea586dd5d48a0aec96cc377dd0e31,
title = "Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray",
abstract = "Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.",
keywords = "Former Faculty of Pharmaceutical Sciences",
author = "Skottrup, {Peter Durand} and Hansen, {Mikkel Fougt} and Moresco, {Jacob Lange} and Maria Deryabina and Svendsen, {Winnie Edith} and Jakobsen, {Mogens Havsteen} and Martin Dufva",
year = "2010",
language = "English",
volume = "6",
pages = "3936--3946",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

AU - Skottrup, Peter Durand

AU - Hansen, Mikkel Fougt

AU - Moresco, Jacob Lange

AU - Deryabina, Maria

AU - Svendsen, Winnie Edith

AU - Jakobsen, Mogens Havsteen

AU - Dufva, Martin

PY - 2010

Y1 - 2010

N2 - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.

AB - Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.

KW - Former Faculty of Pharmaceutical Sciences

M3 - Journal article

VL - 6

SP - 3936

EP - 3946

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 10

ER -

ID: 33238365