Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Peter Durand Skottrup
  • Mikkel Fougt Hansen
  • Jacob Lange Moresco
  • Maria Deryabina
  • Winnie Edith Svendsen
  • Mogens Havsteen Jakobsen
  • Martin Dufva
Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
Bidragets oversatte titelSuperparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray
TidsskriftActa Biomaterialia
Udgave nummer10
Sider (fra-til)3936-3946
Antal sider11
StatusUdgivet - 2010

ID: 33238365