Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group. / Parson, Walther; Niederstätter, Harald; Lindinger, Alexandra; Gill, Peter; Morling, Niels.

I: Forensic Science International: Genetics, Bind 2, Nr. 3, 2008, s. 238-42.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Parson, W, Niederstätter, H, Lindinger, A, Gill, P & Morling, N 2008, 'Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group', Forensic Science International: Genetics, bind 2, nr. 3, s. 238-42. https://doi.org/10.1016/j.fsigen.2007.12.004

APA

Parson, W., Niederstätter, H., Lindinger, A., Gill, P., & Morling, N. (2008). Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group. Forensic Science International: Genetics, 2(3), 238-42. https://doi.org/10.1016/j.fsigen.2007.12.004

Vancouver

Parson W, Niederstätter H, Lindinger A, Gill P, Morling N. Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group. Forensic Science International: Genetics. 2008;2(3):238-42. https://doi.org/10.1016/j.fsigen.2007.12.004

Author

Parson, Walther ; Niederstätter, Harald ; Lindinger, Alexandra ; Gill, Peter ; Morling, Niels. / Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group. I: Forensic Science International: Genetics. 2008 ; Bind 2, Nr. 3. s. 238-42.

Bibtex

@article{a83bbb306bd011de8bc9000ea68e967b,
title = "Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group",
abstract = "The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.",
keywords = "Faculty of Health and Medical Sciences",
author = "Walther Parson and Harald Niederst{\"a}tter and Alexandra Lindinger and Peter Gill and Niels Morling",
note = "Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling",
year = "2008",
doi = "10.1016/j.fsigen.2007.12.004",
language = "English",
volume = "2",
pages = "238--42",
journal = "Forensic Science International: Genetics",
issn = "1872-4973",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

AU - Parson, Walther

AU - Niederstätter, Harald

AU - Lindinger, Alexandra

AU - Gill, Peter

AU - Morling, Niels

N1 - Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling

PY - 2008

Y1 - 2008

N2 - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.

AB - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.

KW - Faculty of Health and Medical Sciences

U2 - 10.1016/j.fsigen.2007.12.004

DO - 10.1016/j.fsigen.2007.12.004

M3 - Journal article

C2 - 19083827

VL - 2

SP - 238

EP - 242

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

SN - 1872-4973

IS - 3

ER -

ID: 13059814