Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.
Originalsprog | Engelsk |
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Tidsskrift | Cell and Tissue Kinetics |
Vol/bind | 15 |
Udgave nummer | 2 |
Sider (fra-til) | 157-168 |
Antal sider | 12 |
ISSN | 0008-8730 |
DOI | |
Status | Udgivet - mar. 1982 |
ID: 203011424