Abundance of ClC-1 chloride channel in human skeletal muscle: Fibre type specific differences and effect of training
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Abundance of ClC-1 chloride channel in human skeletal muscle : Fibre type specific differences and effect of training. / Thomassen, Martin; Hostrup, Morten; Murphy, Robyn M; Cromer, Brett A; Skovgaard, Casper; Gunnarsson, Thomas Petursson; Christensen, Peter Møller; Bangsbo, Jens.
In: Journal of Applied Physiology, Vol. 125, No. 2, 2018, p. 470-478.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Abundance of ClC-1 chloride channel in human skeletal muscle
T2 - Fibre type specific differences and effect of training
AU - Thomassen, Martin
AU - Hostrup, Morten
AU - Murphy, Robyn M
AU - Cromer, Brett A
AU - Skovgaard, Casper
AU - Gunnarsson, Thomas Petursson
AU - Christensen, Peter Møller
AU - Bangsbo, Jens
N1 - CURIS 2018 NEXS 299
PY - 2018
Y1 - 2018
N2 - Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.
AB - Cl- channel protein 1 (ClC-1) may be important for excitability and contractility in skeletal muscle, but ClC-1 abundance has not been examined in human muscle. The aim of the present study was to examine ClC-1 abundance in human skeletal muscle, including fibre type specific differences and the effect of exercise training. A commercially available antibody was tested with positive and negative control tissue, and it recognised specifically ClC-1 in the range from 100-150 kDa. Abundance of ClC-1 was 38% higher (P < 0.01) in fast twitch Type IIa muscle fibres than in slow twitch Type I. Muscle ClC-1 abundance did not change with four weeks of training consisting of 30 min cycling at 85% of maximal heart rate (HRmax) and 3×30-s all out sprints or during a 7-week training period with 10-12×30 s uphill cycling and 4-5×~4 min cycling at 90-95% of HRmax. ClC-1 abundance correlated negatively (P < 0.01) with maximal oxygen consumption (r = -0.552) and incremental exercise performance (r = -0.546). In addition, trained cyclists had lower (P < 0.01) ClC-1 abundance than lesser trained individuals. The present observations indicate that a low abundance of muscle ClC-1 may be beneficial for exercise performance, but the role of abundance and regulation of ClC-1 in skeletal muscle of humans with respect to exercise performance and trainability need to be elucidated.
KW - Faculty of Science
KW - Single fibre
KW - Excitability
KW - Performance
KW - Antibody specificity
KW - ADR
KW - Arrested development of righting response
KW - Proteine expression
KW - Single fiber
U2 - 10.1152/japplphysiol.01042.2017
DO - 10.1152/japplphysiol.01042.2017
M3 - Journal article
C2 - 29722626
VL - 125
SP - 470
EP - 478
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 2
ER -
ID: 196202784