Dried urine swabs as a tool for monitoring metabolite excretion
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Dried urine swabs as a tool for monitoring metabolite excretion. / Monošík, Rastislav; Dragsted, Lars Ove.
In: Bioanalysis, Vol. 10, No. 17, 2018, p. 1371-1382.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Dried urine swabs as a tool for monitoring metabolite excretion
AU - Monošík, Rastislav
AU - Dragsted, Lars Ove
N1 - CURIS 2018 NEXS 274
PY - 2018
Y1 - 2018
N2 - Aim: We tested a large set (n = 181) of wet urine and dried urine samples spotted on regular cosmetic cotton swabs for comparative UHPLC-MSMS analysis of various metabolites across a wide polarity and structural range. Results/methodology: The agreement of measurements between conventional 24 h urines and dried urine spots made from them in situ was evaluated by Passing-Bablok regression and Bland-Altman analysis after creatinine correction. There was full agreement in qualitative results but quantitative analysis revealed underestimation of dried urine spots in some cases. The dried urine samples contained analytes at measurable levels for at least 9 months.Conclusion: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.
AB - Aim: We tested a large set (n = 181) of wet urine and dried urine samples spotted on regular cosmetic cotton swabs for comparative UHPLC-MSMS analysis of various metabolites across a wide polarity and structural range. Results/methodology: The agreement of measurements between conventional 24 h urines and dried urine spots made from them in situ was evaluated by Passing-Bablok regression and Bland-Altman analysis after creatinine correction. There was full agreement in qualitative results but quantitative analysis revealed underestimation of dried urine spots in some cases. The dried urine samples contained analytes at measurable levels for at least 9 months.Conclusion: Although this technique seems very promising more methodological studies have to be conducted in order to improve applicability of dried urine microvolume fluidic sampling for quantitative analysis.
KW - Faculty of Science
KW - UHPLC-MS/MS
KW - Creatinine correction
KW - Dried urine spots
U2 - 10.4155/bio-2018-0042
DO - 10.4155/bio-2018-0042
M3 - Journal article
C2 - 30088416
VL - 10
SP - 1371
EP - 1382
JO - Bioanalysis
JF - Bioanalysis
SN - 1757-6180
IS - 17
ER -
ID: 201043993