Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle
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Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle. / Birk, Jesper Bratz; Wojtaszewski, Jørgen.
AMPK: Methods and Protecols. ed. / Dietbert Neumann; Benoit Viollet. New York : Humana Press, 2018. p. 215-228 (Methods in Molecular Biology; No. 1732).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle
AU - Birk, Jesper Bratz
AU - Wojtaszewski, Jørgen
N1 - CURIS 2018 NEXS 080
PY - 2018
Y1 - 2018
N2 - Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.
AB - Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.
KW - Faculty of Science
KW - Immunoprecipitation
KW - AMPK activity
KW - Skeletal muscle
KW - Isoform-specific
KW - Radioactive tracer 33P
KW - AMARA peptide
U2 - 10.1007/978-1-4939-7598-3_14
DO - 10.1007/978-1-4939-7598-3_14
M3 - Book chapter
C2 - 29480478
SN - 978-1-4939-7597-6
T3 - Methods in Molecular Biology
SP - 215
EP - 228
BT - AMPK
A2 - Neumann, Dietbert
A2 - Viollet, Benoit
PB - Humana Press
CY - New York
ER -
ID: 191294168