TY - JOUR

T1 - Changes in S100 Proteins Identified in Healthy Skin following Electrical Stimulation

T2 - Relevance for Wound Healing

AU - Lallyett, Chloe

AU - Yeung, Ching-Yan Chloé

AU - Nielson, Rie Harboe

AU - Zeef, Leo A H

AU - Chapman-Jones, David

AU - Kjaer, Michael

AU - Kadler, Karl E

PY - 2018

Y1 - 2018

N2 - OBJECTIVE: Targeted electrical energy applied to wounds has been shown to improve wound-healing rates. However, the mechanisms are poorly understood. The aim of this study was to identify genes that are responsive to electrical stimulation (ES) in healthy subjects with undamaged skin.METHODS: To achieve this objective, study authors used a small, noninvasive ES medical device to deliver a continuous, specific, set sequence of electrical energy impulses over a 48-hour period to the skin of healthy volunteers and compared resultant gene expression by microarray analysis.MAIN RESULTS: Application of this specific ES resulted in differential expression of 105 genes, the majority of which were down-regulated. Postmicroarray analyses revealed there was commonality with a small number of genes that have previously been shown to be up-regulated in skin wounds, including venous leg ulcers.CONCLUSIONS: The specific sequence of ES applied continuously for 48 hours to the skin of healthy patients has the effect of modifying expression in a number of identified genes. The identification of the differential expression in this subset of genes in healthy subjects provides new potential lines of scientific inquiry for identifying similar responses in subjects with slow or poorly healing wounds.

AB - OBJECTIVE: Targeted electrical energy applied to wounds has been shown to improve wound-healing rates. However, the mechanisms are poorly understood. The aim of this study was to identify genes that are responsive to electrical stimulation (ES) in healthy subjects with undamaged skin.METHODS: To achieve this objective, study authors used a small, noninvasive ES medical device to deliver a continuous, specific, set sequence of electrical energy impulses over a 48-hour period to the skin of healthy volunteers and compared resultant gene expression by microarray analysis.MAIN RESULTS: Application of this specific ES resulted in differential expression of 105 genes, the majority of which were down-regulated. Postmicroarray analyses revealed there was commonality with a small number of genes that have previously been shown to be up-regulated in skin wounds, including venous leg ulcers.CONCLUSIONS: The specific sequence of ES applied continuously for 48 hours to the skin of healthy patients has the effect of modifying expression in a number of identified genes. The identification of the differential expression in this subset of genes in healthy subjects provides new potential lines of scientific inquiry for identifying similar responses in subjects with slow or poorly healing wounds.

KW - Electric Stimulation/methods

KW - Healthy Volunteers

KW - Humans

KW - S100 Proteins/physiology

KW - Skin/injuries

KW - Wound Healing/physiology

U2 - 10.1097/01.ASW.0000533722.06780.03

DO - 10.1097/01.ASW.0000533722.06780.03

M3 - Journal article

C2 - 29923902

VL - 31

SP - 322

EP - 327

JO - Advances in Skin and Wound Care

JF - Advances in Skin and Wound Care

SN - 1527-7941

IS - 7

ER -