Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans
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Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids : a method applicable in humans. / Holm, Lars; O'Rourke, Bruce; Ebenstein, David; Toth, Michael J; Bechshoeft, Rasmus; Holstein-Rathlou, Niels-Henrik; Kjær, Michael; Matthews, Dwight E.
I: A J P: Endocrinology and Metabolism (Online), Bind 304, Nr. 8, 2013, s. E895-E907.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids
T2 - a method applicable in humans
AU - Holm, Lars
AU - O'Rourke, Bruce
AU - Ebenstein, David
AU - Toth, Michael J
AU - Bechshoeft, Rasmus
AU - Holstein-Rathlou, Niels-Henrik
AU - Kjær, Michael
AU - Matthews, Dwight E
PY - 2013
Y1 - 2013
N2 - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.
AB - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.
U2 - 10.1152/ajpendo.00579.2012
DO - 10.1152/ajpendo.00579.2012
M3 - Journal article
VL - 304
SP - E895-E907
JO - A J P: Endocrinology and Metabolism (Online)
JF - A J P: Endocrinology and Metabolism (Online)
SN - 1522-1555
IS - 8
ER -
ID: 119412018