Effects of ageing on single muscle fibre contractile function following short-term immobilisation
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Effects of ageing on single muscle fibre contractile function following short-term immobilisation. / Hvid, Lars G; Ortenblad, Niels; Aagaard, Per; Kjaer, Michael; Suetta, Charlotte.
I: Journal of Physiology, Bind 589, Nr. Pt 19, 2011, s. 4745-57.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effects of ageing on single muscle fibre contractile function following short-term immobilisation
AU - Hvid, Lars G
AU - Ortenblad, Niels
AU - Aagaard, Per
AU - Kjaer, Michael
AU - Suetta, Charlotte
PY - 2011
Y1 - 2011
N2 - Very little attention has been given to the combined effects of healthy ageing and short-term disuse on the contractile function of human single muscle fibres. Therefore, the present study investigated the effects of 2 weeks of lower limb cast immobilisation (i.e. disuse) on selected contractile properties of single muscle fibres (n = 378) from vastus lateralis of nine young (24 ± 1 years) and eight old (67 ± 2 years) healthy men with comparable levels of physical activity. Prior to immobilisation, MHC IIa fibres produced higher maximum Ca(2+)-activated force (approx. 32%) and specific force (approx. 33%) and had lower Ca(2+) sensitivity than MHC I fibres (P <0.05), with no differences between young and old. After immobilisation, the decline in single fibre force (MHC I: young 21% and old 22%; MHC IIa: young 22% and old 30%; P <0.05) as well as specific force (MHC I: young 14% and old 13%; MHC IIa: young 18% and old 25%; P <0.05) was more pronounced in MHC IIa fibres compared to MHC I fibres (P <0.05), with no differences between young and old. Notably, there was a selective decrease in Ca(2+) sensitivity in MHC IIa fibres of young (P <0.05) and in MHC I fibres of old individuals (P <0.05), respectively. In conclusion, 2 weeks of lower limb immobilisation caused greater impairments in single muscle fibre force and specific force in MHC IIa than MHC I fibres independently of age. In contrast, immobilisation-induced changes in Ca(2+) sensitivity that were dependent on age and MHC isoform.
AB - Very little attention has been given to the combined effects of healthy ageing and short-term disuse on the contractile function of human single muscle fibres. Therefore, the present study investigated the effects of 2 weeks of lower limb cast immobilisation (i.e. disuse) on selected contractile properties of single muscle fibres (n = 378) from vastus lateralis of nine young (24 ± 1 years) and eight old (67 ± 2 years) healthy men with comparable levels of physical activity. Prior to immobilisation, MHC IIa fibres produced higher maximum Ca(2+)-activated force (approx. 32%) and specific force (approx. 33%) and had lower Ca(2+) sensitivity than MHC I fibres (P <0.05), with no differences between young and old. After immobilisation, the decline in single fibre force (MHC I: young 21% and old 22%; MHC IIa: young 22% and old 30%; P <0.05) as well as specific force (MHC I: young 14% and old 13%; MHC IIa: young 18% and old 25%; P <0.05) was more pronounced in MHC IIa fibres compared to MHC I fibres (P <0.05), with no differences between young and old. Notably, there was a selective decrease in Ca(2+) sensitivity in MHC IIa fibres of young (P <0.05) and in MHC I fibres of old individuals (P <0.05), respectively. In conclusion, 2 weeks of lower limb immobilisation caused greater impairments in single muscle fibre force and specific force in MHC IIa than MHC I fibres independently of age. In contrast, immobilisation-induced changes in Ca(2+) sensitivity that were dependent on age and MHC isoform.
U2 - http://dx.doi.org/10.1113/jphysiol.2011.215434
DO - http://dx.doi.org/10.1113/jphysiol.2011.215434
M3 - Journal article
VL - 589
SP - 4745
EP - 4757
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - Pt 19
ER -
ID: 40170984