Bovine cumulus-oocyte disconnection in vitro
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Bovine cumulus-oocyte disconnection in vitro. / Maddox-Hyttel, Poul.
I: Brain Structure and Function (Print Edition), Bind 176, 1987, s. 41-44.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Bovine cumulus-oocyte disconnection in vitro
AU - Maddox-Hyttel, Poul
PY - 1987
Y1 - 1987
N2 - Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
AB - Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
KW - Former LIFE faculty
KW - Bovine
KW - Oocyte maturation
KW - Meiosis
KW - Gap junction
KW - Ultrastructure
M3 - Journal article
VL - 176
SP - 41
EP - 44
JO - Brain Structure and Function
JF - Brain Structure and Function
SN - 1863-2653
ER -
ID: 8067538